The Effect of Giving Croton Leaf Crude Extract (Codiaeum variegatum) as an Alternative to The Antibacterial of Edwardsiella tarda in Vitro

Production problems related to aquaculture cannot be separated from diseases that can cause death in fish. Efforts overcome the bacterial disease Edwardsiella tarda done by using natural materials such as leaves of croton (Codiaeum variegatum). This study aimed analyze content active compounds contained leaf extract which is antibacterial against E. bacteria. Inhibition test was performed dilution method Minimum Concentration (MIC test) and agar diffusion (disc test). The MIC used five concentrations: 1 mg/L, 10 100 500 1000 mg/L on Tryptone Soy Broth (TSB) media, while disc doses extract, namely: A (100 mg/L), B ( 200 C (300 D (400 mg/L) E (500 2 controls (positive negative), three replications Agar (TSA) media incubated for 2x24 h. positive control chloramphenicol (5 antibiotic. Croton contains several compounds, namely saponins, flavonoids, tannins, steroids, polyphenols, are could kill bacteria with highest average inhibition zone results obtained at a dose 6.34 mm, lowest were 3.12 mm.